Description:
HapiCell? Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity. HapiCell? Transfection Toxicity Removal Cocktail was developed to contain a mixture of several chemicals which were confirmed to effectively remove DNA/siRNA transfection mediated cytotoxicity. Incubation of sensitive cells (e.g., SaoS-2, HUVEC, primary kerotinocytes, etc) with HapiCell Toxicity Removal Reagent for 2 minutes at RT was confirm to completely remove the transfection related toxicity which otherwise kill the cells. The cocktail was used for chemical transfection only, not for electroporation. HapiCell? Transfection Toxicity Removal Cocktail, 2.5 ml (x50 stock solution), is sufficient to treat 125 wells for 24-well plate and 63 wells for 6-well plate respectively.
Feature:
– Removes transfection mediated toxicity after 2 minutes incubation at RT
– Nontoxic to cells
– Maintain cell viability
– Improve transfection efficiency
– Easy to use
HapiCell? Transfection Toxicity Removal Cocktail Effectively Remove Transfection Mediated Toxocity:
A example showing complete removal of transfection toxicity after treatment with HapiCell? Transfection Toxicity Removal Cocktail on mouse skin fibroblast. A DNA encoding GFP was delivered to mouse skin fibroblasts by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (left panel) or without (right panel) HapiCell? Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection
A example showing complete removal of transfection toxicity after treatment with HapiCell? Transfection Toxicity Removal Cocktail on primary mouse kerotinocytes. A DNA encoding GFP was delivered to primary mouse kerotinocytes by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (left panel) or without (right panel) HapiCell? Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection
