Contents Per Kit: ● 1×1.0 ml of GenJet? DNA Transfection Reagent
● 1×1.0 ml (x50) of HapiCell? Transfection Toxicity Removal Cocktail
Description GenJet? DNA In Vitro Tranfection Reagent for SMC is pre-optimized for transfecting smooth muscle cells. Smooth muscle cells are notoriously hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell? Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 75% efficiency.
Refer to the following optimal transfection conditions for maximal transfection efficiency on Hela cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
95%
Cell culture conditions
DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio
3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex
15 minutes at RT
Presence of Serum/Antibiotics during Transfection
Yes
Change Medium After Transfection
Yes, ~5 Hours After Transfection
Treatment with HapiCell? Toxicity Removal Cocktail
Yes, ~5 Hours After Transfection Followed by PBS Wash
Maximal Efficiency
48 hours
Transfection Results:
Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %)
55%
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer Data Sheet
